Generation of heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2/COUP-TFII knockout human iPSC lines

Generation of heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2/COUP-TFII knockout human iPSC lines

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The NR2F2 gene encodes the transcription factor COUP-TFII, which is upregulated in embryonic mesoderm.Heterozygous variants in NR2F2 cause a spectrum of congenital anomalies including cardiac and gonadal phenotypes.We generated heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2-knockout induced pluripotent stem cell (iPSC) lines from cottonmouth 3.5 high shirt human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming.Both iPSC lines exhibited a normal karyotype, typical pluripotent cell morphology, pluripotency marker expression, and the capacity to differentiate into the three embryonic germ layers.These lines will allow us to explore yamaha c115v specs the role of NR2F2 during development and disease.